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Alternatively, the stock lifestyle organisms might be grown in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) as well as the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to acquire a microbial count of about one × 108 cfu for every mL. [Take noteThe esti